HPLC法测定地黄中地黄苷D含量

目的　建立地黄中地黄苷 D的 HPLC测定方法 ,提出地黄苷 D的含量限度。方法　 Hypersil C1 8分析柱(2 5 0 mm× 4.6mm,5μm) ,流动相为乙腈 -水 (4∶ 96) ,流速为 1m L /m in,柱温为室温 ,检测波长为 2 0 5 nm。结果　地黄苷 D回收率为 10 0 .6% ,RSD为 2 .1% ,8个产地的 16份完整药材中地黄苷 D的含量为 0 .13 7%～ 0 .691%。结论　方法快速、简便、准确 ,可用于地黄质量控制 ,建议地黄苷 D的含量应不低于 0 .15 %。     

间苯三酚与硫酸镁治疗先兆早产的临床效果比较

目的比较间苯三酚及硫酸镁治疗先兆早产的临床效果。方法对60例诊断为先兆早产的孕妇作为研究组,给予间苯三酚治疗,并选择43例符合相同条件的病例作为对照组,给予硫酸镁治疗。结果应用间苯三酚及硫酸镁治疗治疗能使有效率分别达90.0%和88.4%,延长孕周至37周数达83.3%和83.7%,两者疗效比较无显著性差异（P〉0.05）,但间苯三酚副作用较硫酸镁少。结论间苯三酚能有效抑制宫缩,是治疗先兆早产有效而且安全和方便的方法。     

闭角性青光眼小梁细胞凋亡探讨

目的:研究闭角性青光眼小梁细胞凋亡发生率与眼压升高的关系。方法:取小梁组织染色固定,行电镜观察。结果:眼压<5.48kPa时,小梁细胞的形态正常,眼压>5.48kPa时出现变性的小梁细胞。结论:闭角性青光眼小梁细胞凋亡明显增加,与眼压升高成正相关。     

5-氮脱氧胞苷及曲古抑菌素A影响供核细胞H19基因表达的时间依赖性及浓度依赖性

目的:探讨甲基转移酶抑制剂——5-氮脱氧胞苷(5-aza-2′-deoxycytidine,5-aza-dC)和组蛋白去乙酰基酶抑制剂——曲古抑菌素A(trichostatin A,TSA)影响供核细胞H19基因表达的时间依赖性和浓度依赖性。方法:用不同浓度5-aza-dC及TSA按不同作用时间处理供核细胞(鼠尾尖成纤维样细胞),利用实时定量PCR法检测处理后H19基因的表达。结果:不同浓度的5-aza-dC处理组之间H19的表达无明显差异,但延长作用时间可使H19表达增强;TSA使H19的表达下降,但无时间依赖性。结论:长时间低浓度的5-aza-dC能增强供核细胞中H19基因的表达,而TSA则使H19的表达减弱,为体细胞克隆中表观遗传修饰抑制剂的选择及后续基因印迹研究提供了依据。     

Abrogation of ARF6 in promoting erastin-induced ferroptosis and mitigating capecitabine resistance in gastric cancer cells

BackgroundADP ribosylation factor 6 (ARF6) is a member of the Rat sarcoma virus (RAS) superfamily that is involved in the regulation of vesicular trafficking, membrane lipid remodeling, and signaling pathways. Our earlier work discovered that ARF6, as a downstream effector of the Kirsten rat sarcoma viral oncogene (Kras)/extracellular signal-regulated kinases (ERK) signaling pathway, may increase proliferation and induce the Warburg effect in gastric cancer (GC) cells. Additionally, ARF6 appears to be a potential biomarker for predicting the prognosis of GC. Ferroptosis has recently been described as a type of nonapoptotic iron-dependent cell death that is strongly associated with the Kras mutation. Therefore, it is critical to continue investigating the link between ARF6 and ferroptosis.MethodsWe first created ARF6 silenced cancer cell lines with lentivirus transfection. The knockdown efficiency was confirmed through quantitative polymerase chain reaction (qPCR) and western blotting. Subsequently, we used Cell Counting Kit-8 (CCK-8) and malondialdehyde (MDA) assay for lipid peroxidation measurement. Following this, qPCR and western blotting were conducted to clarify the mechanism involved. Finally, immunohistochemistry was used to stain human GC samples.ResultsOur findings established that, whereas ARF6 did not directly regulate lipid peroxidation, it did render GC cells susceptible to oxidative stress, particularly erastin-induced lipid peroxidation. Additionally, our research demonstrated that ARF6 may control capecitabine resistance via several routes.ConclusionsARF6 may play a critical role in the development of GC.     

Survival and prognostic factors for postoperative primary appendiceal cancer: a retrospective cohort study based on the Surveillance,Epidemiology, and End Results database

BackgroundThe factors affecting the postoperative survival of patients with primary appendiceal cancer (PAC) have yet to be fully explored. And there are no clear guidelines for adjuvant treatment after appendectomy. Whether chemotherapy can prolong patient survival after appendectomy, is critical in guiding postoperative medications. The majority of studies on appendiceal cancer are single case reports, and they focused on the incidence of appendiceal cancer. The present study aimed to investigate the survival characteristics of patients with primary appendiceal cancer after surgery using the Surveillance, Epidemiology, and End Results (SEER) database.MethodsThe data of 2,891 cases of primary appendiceal cancer between 2004 to 2015 were obtained from the SEER database and subjected to survival analysis using the Kaplan-Meier method and Cox proportional-hazards model. The annual percentage change (APC) was calculated using the weighted least squares method.ResultsThe overall age-adjusted incidence rate per 100,000 population steadily increased from 0.58 in 2004 to 1.63 in 2015. For patients who received chemotherapy, the median overall survival (OS) was 65 months and the 5-year OS rate was 51.9%, and for patients who did not receive chemotherapy or whose chemotherapy status was unknown, the median OS was not reached and the 5-year OS rate was 78.9%. Age [35< age <69: hazard radio (HR) =2.147; 95% confidence interval (CI): 1.442–3.197, P<0.001; age >69: HR =5.259; 95% CI: 3.485–7.937, P<0.001], race (White race: HR =0.728; 95% CI: 0.590–0.899, P=0.003), histologic type (mucinous neoplasm: HR =0.690; 95% CI: 0.580–0.821, P<0.001; malignant carcinoid: HR =0.657; 95% CI: 0.536–0.806, P<0.001), grade (II: HR =1.794; 95% CI: 1.471–2.187, P<0.001; III: HR =2.905; 95% CI: 2.318–3.640, P<0.001; IV: HR =3.128; 95% CI: 2.159–4.533, P<0.001), and stage (localized: HR =0.236; 95% CI: 0.194–0.287, P<0.001; regional: HR =0.425; 95% CI: 0.362–0.499, P<0.001) were identified as independent predictors of survival. And after adjusting for known factors (age, sex, race, tumor size, marital status, histologic type, grade, stage), chemotherapy (HR =1.220; 95% CI: 1.050–1.417, P=0.009) was revealed to be an independent indicator of poor prognosis.ConclusionsThere was an increasing trend in the incidence of appendiceal cancer in the United States between 2004 and 2015. Chemotherapy was revealed to be an independent indicator of poor prognosis, which provide valuable insight into the therapy of primary appendiceal cancer. Large clinical trials of chemotherapy and targeted therapy for appendiceal cancer are urgently needed.     

高原地区青少年无氧代谢阈值的特征及其影响因素的探讨

目的探讨不同海拔低氧环境对移居青少年无氧代谢阈值的影响。方法使用Jae-ger自行车功量仪,心阻抗仪,用逐级运动负荷法分别测定移居海拔2 260 m、3 417 m和4 280 m三个海拔高度,13~16岁青少年的无氧代谢阈值(AT),同步测定动脉血氧饱和度(SaO2)和心输出量(CO),分析其间的相互关系。结果AT值随海拔高度的上升而下降,以氧耗量表示,分别为(27.44±6.50)、(22.88±5.38)和(16.95±5.97)m l/m in.kg。AT值的降低与SaO2和CO的降低呈正相关。结论SaO2和CO是影响AT值的重要因素。长期移居高海拔的青少年AT值降低,海拔越高,降低越明显。     

博心通软胶囊的制备工艺考察

目的：研究博心通软胶囊的制备工艺。方法：采用CO2超临界流体萃取技术对葱白有效部位进行提取;以胶囊内容物的流动性、均匀性、沉降体积比及囊壳的软硬度,粘连性等为指标,确定软胶囊处方及制备工艺。结果：胶囊内容物的处方为葱白提取物：PEG400：丙二醇：吐温80：聚维酮K30=10：20：1：0．2：0．6（w／w）,囊壳处方为明胶：甘油：水：氧化铁：尼泊金乙酯：1：0．4：1：适量：适量（w／w）。结论：所确定的处方工艺稳定可靠,重复性好,适合工业生产。     

UPLC(超高效液相色谱)/MS/MS联用技术测定全血中的西罗莫司

目的 建立全血中西罗莫司的UPLC（超高效液相色谱）／MS／MS测定方法,方法 全血样品中加入他克莫司（FK506）作内标，用叔丁基甲醚进行提取。以乙腈与含千分之五甲酸的水溶液为流动相（40：50），色谱柱为AcquityUPLCTMBEHC。（50mm×2．1mm，1．7μm），流速0．5ml／min。三重四极杆质谱采用正离子模式，离子采集方式为多反应监测模式（MRM），离子源温度105℃，离子源电离电压为3300V，雾化气流速500L／h。采集离子（母离子／子离子）西罗莫司为931．2／864．1，FK506为822．0／577．0。结果 西罗莫司在1～240腿／L浓度范围内呈良好的线性（n=0．9995）。日内、日间精密度均在15％以内，提取回收率大于75．0％，方法回收率为95．0％～98．5％，最低检测限为0．2μg／L。结论 本方法灵敏、准确，适合临床西罗莫司的全血分析。     

利用近红外图谱比对法及一致性检验模型鉴别健胃消食片

目的 在不破坏药品包装的情况下,利用近红外光谱快速鉴别健胃消食片(江中药业股份有限公司).方法 在12000～4000 cm-1波段范围内,对同一厂家的健胃消食片进行全谱扫描,通过图谱比对,建立一致性检验模型.结果 正品健胃消食片与伪品健胃消食片的近红外光谱在5500～9000 cm-1波长范围内存在较大差别,通过建立该品种的一致性近红外模型,可对健胃消食片进行初筛.结论 该方法快速简便、准确有效,为基层打假提供有效方法.